The University of Pittsburgh, Department of Immunology, is seeking a full-time technician to support newly funded research programs. The Elsner lab investigates immune responses during infection and autoimmune disease, focusing primarily on B cell responses (see Elsner et al Nature Immunology 2024; Sanz, Nature Immunology 2024; Elsner and Shlomchik, Immunity 2020; Elsner and Shlomchik, Immunological Reviews 2025). There are opportunities for independent projects within the mission of the laboratory, based on the applicant’s skills and goals. Applicants that are interested in having their own independent project are encouraged to apply.

The applicant will support the function of the laboratory and assist scientists conducting experiments in cellular and molecular immunology. The applicant should have experience with flow cytometry and basic immunological techniques. A significant task will include maintenance of the mouse colony, which includes breeding, PCR-based genotyping, and record keeping. The applicant should be very eager to learn new techniques and improve existing techniques.

Qualifications: A Bacherlor’s Degree in Immunology, Biology or related field plus two (2) Years of Professional Years of Experience is required in immunology/microbiology/molecular biology or other related fields. Knowledge of mathematical and chemical principles. Must be highly organized and efficient with their time. Must maintain professional demeanor and work well with others. Must be able to prioritize daily duties.

Performs a variety of independent laboratory work and experiments such as determining experimental protocols and preparation of specimens and cultures. Prepares reports of findings subsequent to compilation and analyzation. Oversees specimen inventory. Develops documentation for publication; crafts manuscripts and grant proposals.

Performs a variety of independent laboratory work and experiments such as determining experimental protocols and preparation of specimens and cultures. Prepares reports of findings subsequent to compilation and analyzation. Oversees specimen inventory. Develops documentation for publication; crafts manuscripts and grant proposals.

Responsibilities for this position include:

1. Perform independent laboratory work and experiments, including analysis and interpretation of murine and human cell culture experiments. These will involve cell isolation from organs, immunofluorescence staining, flow cytometry, ELISA/ELISpot, cell sorting, and short-term cell culture. Writes reports and summaries of findings.

2. Oversees specimen inventories.

3. Maintains lab and chemical inventories. Assists with ordering as needed.

4. Prepares data for presentation to the group and for publication.

5. Assist with training of other lab members in basic immunological procedures.